Stanley, Shawn M. R. and Chua, Danny (2014) Improved Recovery of Erythropoietin and Darbepoetin from Equine Plasma by the Application of a Wheat Germ Agglutinin Mediated Pre-Extraction Prior to Immunoaffinity Chromatography. Advances in Bioscience and Biotechnology, 05 (07). pp. 651-660. ISSN 2156-8456
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Abstract
We describe a two-step method that uses wheat germ agglutinin immobilized on Sepharose gel followed by immunoaffinity chromatography (IAC) to extract recombinant human erythropoietin and Darbepoetin from equine plasma. Lectin affinity chromatography was shown to be an effective approach for isolating these epoetins from plasma and in combination with IAC; this method gave superior recovery when compared to the use of the latter technique alone. Moreover, due to the ease with which it can be scaled up, it is particularly well suited for pre-concentrating larger volumes of samples prior to IAC and this provides a facile way of improving the overall sensitivity with which these foreign proteins can be detected in equine plasma.
Item Type: | Article |
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Subjects: | Open Library Press > Biological Science |
Depositing User: | Unnamed user with email support@openlibrarypress.com |
Date Deposited: | 11 Mar 2023 08:56 |
Last Modified: | 03 Sep 2024 04:52 |
URI: | http://info.euro-archives.com/id/eprint/725 |